• Product NameSTUB1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG38-22
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,IP,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within human STUB1 aa 1-200.
• Alternative Names    Antigen NY CO 7 antibody
  Antigen NY-CO-7 antibody
  C terminus of Hsp70-interacting protein antibody
  Carboxy terminus of Hsp70 interacting protein antibody
  Carboxy terminus of Hsp70-interacting protein antibody
  Carboxy terminus of Hsp70p interacting protein antibody
  CHIP antibody
  CHIP_HUMAN antibody
  CLL associated antigen KW 8 antibody
  CLL-associated antigen KW-8 antibody
  E3 ubiquitin protein ligase CHIP antibody
  E3 ubiquitin-protein ligase CHIP antibody
  Heat shock protein A binding protein 2 (c terminal) antibody
  HSPABP2 antibody
  NY CO 7 antibody
  PP1131 antibody
  SDCCAG7 antibody
  Serologically defined colon cancer antigen 7 antibody
  STIP1 homology and U Box containing protein 1 antibody
  STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
  STIP1 homology and U box-containing protein 1 antibody
  STUB 1 antibody
  STUB1 antibody
  UBOX 1 antibody
  UBOX1 antibody
  
• Accession No. Swiss-Prot#:Q9UNE7
• UniprotQ9UNE7
• Gene ID10273;
• Calculated MW 35 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  IP: 1:10-1:50
  FC: 1:50-1:100

CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain.

• Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.
  Positive control:
  Lane 1: 293
  Lane 2: MCF-7
  Lane 3: SK-Br-3
  Lane 4: Mouse kidney
  
• Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
• ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting