• Product NameIMPDH2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JE40-87
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC,IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within C-terminal human IMPDH2.
• Alternative Names    IMDH2_HUMAN antibody
  IMP (inosine monophosphate) dehydrogenase 2 antibody
  IMP dehydrogenase 2 antibody
  IMP oxireductase 2 antibody
  IMPD 2 antibody
  IMPD2 antibody
  IMPDH 2 antibody
  IMPDH II antibody
  IMPDH-II antibody
  Impdh2 antibody
  Inosine 5' monophosphate dehydrogenase 2 antibody
  Inosine 5' phosphate dehydrogenase 2 antibody
  Inosine monophosphate dehydrogenase type II antibody
  Inosine-5''-monophosphate dehydrogenase 2 antibody
  
• Accession No. Swiss-Prot#:P12268
• UniprotP12268
• Gene ID3615;
• Calculated MW 56 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  IP: 1:10-1:50
  FC: 1:10-1:50

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

• Western blot analysis of IMPDH2 on Daudi cell lysate using anti-IMPDH2 antibody at 1/2,000 dilution.
• Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
• ICC staining IMPDH2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining IMPDH2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining IMPDH2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Daudi cells with IMPDH2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting