• Product NameFBXL12 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide contain a sequence corresponding to a region within amino acids 265 and 326 of FBXL12
• Target NameFBXL12
• Accession No. Swiss-Prot:Q9NXK8
  Gene ID:54850
• UniprotQ9NXK8
• Gene ID54850;
• Concentration 1mg/ml
• Formulation Supplied in 1XPBS, 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 37kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  

Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

• Sample (30 ug of whole cell lysate)
  A: Hep G2
  10% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL12 antibody at 1: 500 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting