• Product NameENO2/NSE Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Purificationprotein G purifed
• ApplicationsWB IHC
• Species ReactivityHu
• Specificityspecific for Human ENO2 denatured and native forms
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Human ENO2
• Target NameENO2/NSE
• Alternative Names    2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
• Accession No. Swiss-Prot#: P09104
• UniprotP09104
• Gene ID2026;
• Calculated MW 48kd
• Concentration 1.0mg/mL
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

• All Lanes Mouse anti-human ENO2 monoclonal antibody at 1ug/ml Lane 1 Hela cell lysate Lane 2 Recombinant Human Gamma-enolase(ENO2) at 10ug Lane 3 U251 cell lysate Secondary Goat polyclonal to Mouse IgG at 1/3000 dilution Predicted band size : 48 kDa Observed band size: 48kDa Additional bands at : 100kDa. We are unsure as to the identity of this extra band.
• Immunohistochemical analysis of paraffin-embedded human small intestine using #42020 at dilution of 1:200.
• Immunohistochemical analysis of paraffin-embedded human pancreas using #42020 at dilution of 1:200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting