Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LAMA4 in samples. An antibody specific for LAMA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Laminin subunit alpha-4 isalpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Also, alternative splicing involving the first intron in the 5' UTR, and laminin alpha 4 like isoforms have been noted, however, the full-length nature of these products is not known.
