• Product NameCytochrome c oxidase subunit 5B, mitochondrial Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu Ms Rt Cow
• SpecificityThe antibody detects endogenous level of total Cytochrome c oxidase subunit 5B, mitochondrial polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Cytochrome c oxidase subunit 5B, mitochondrial protein
• Target NameCytochrome c oxidase subunit 5B, mitochondrial
• Alternative Names    COX5B
• Accession No. Swiss-Prot#: P10606
• UniprotP10606
• Gene ID1329;
• Calculated MW 14kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

[1] "Sequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase."Zeviani M., Sakoda S., Sherbany A., Nakase H., Rizzuto R., Samitt C.E., Dimauro S., Schon E.A.Gene 65:1-11(1988) [2] "Structure of the human cytochrome c oxidase subuni

• All lanes : Cytochrome c oxidase subunit 5B, mitochondrial antibody at at 2ug/ml Lane 1 : EC109 whole cell lysateLane 2 : 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 14kDa Observed band size: 14 kDa Additional bands at : 65kDa. We are unsure as to the identity of this extra band.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting