• Product NameRas-related GTP-binding protein A Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Ras-related GTP-binding protein A polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Ras-related GTP-binding protein A protein
• Target NameRas-related GTP-binding protein A
• Alternative Names    Adenovirus E3 14.7 kDa-interacting protein 1, RRAGA, FIP-1
• Accession No. Swiss-Prot#: Q7L523
• UniprotQ7L523
• Gene ID10670;
• Calculated MW 34kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Involved in the RCC1/Ran-GTPase pathway. RRAGA may play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. biquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.

[1]"Cloning of a novel family of mammalian GTP-binding proteins (RagA, RagBs, RagBl) with remote similarity to the Ras-related GTPases."Schuermann A., Brauers A., Massmann S., Becker W., Joost H.-G.J. Biol. Chem. 270:28982-28988(1995) [2]

• All lanes : Ras-related GTP-binding protein A antibody at 2ug/ml + 293T whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 34kDa Observed band size: 34kDa
• Immunohistochemical analysis of paraffin-embedded human lungs using #42394 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting