• Product NameProfilin-1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Profilin-1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Profilin-1 protein
• Target NameProfilin-1
• Alternative Names    PFN1
• Accession No. Swiss-Prot#: P07737
• UniprotP07737
• Gene ID5216;
• Calculated MW 15.4kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

[1] "Human profilin. Molecular cloning, sequence comparison, and chromosomal analysis." Kwiatkowski D.J., Bruns G.A.P. J. Biol. Chem. 263:5910-5915(1988) [PubMed: 3356709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] "The status, quality,

• All lanes : Profilin-1 antibody at at 2ug/mlLane 1 : EC109 whole cell lysateLane 2 : 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 15.4 kDa Observed band size: 15.4kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting