• Product NameGlutamate carboxypeptidase 2 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Glutamate carboxypeptidase 2 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Glutamate carboxypeptidase 2 protein
• Target NameGlutamate carboxypeptidase 2
• Alternative Names    GIG27, FOLH, NAALAD1, PSM, PSMA, FOLH1, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate
• Accession No. Swiss-Prot#: Q04609
• UniprotQ04609
• Gene ID2346;
• Calculated MW 82kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

[1]"Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen."IsraeliR.., Powell C.T., Fair W.R., Heston W.D.W.Cancer Res. 53:227-230(1993) [2]"Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio

• All lanes:Glutamate carboxypeptidase 2 antibody at 2ug/ml Lane 1:U251 whole cell lysate Lane 2:PC-3 whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :82kDa observed band size :82kDa
• Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma using #42546 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting