• Product NameMethylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Methylated-DNA--protein-cysteine methyltransferase polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Methylated-DNA--protein-cysteine methyltransferase
• Target NameMethylated-DNA--protein-cysteine methyltransferase
• Alternative Names    MGMT
• Accession No. Swiss-Prot#: P16455
• UniprotP16455
• Gene ID4255;
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:20 - 1:200

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

[1] "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine."Tano K., Shiota S., Collier J., Foote R.S., Mitra S.Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990) [2] "cDNA clo

• Immunohistochemical analysis of paraffin-embedded human kidney using #42551 at dilution of 1:20.
• Immunohistochemical analysis of paraffin-embedded human colon cancer using #42551 at dilution of 1:20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting