• Product NameGTPase ERas Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total GTPase ERas polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human GTPase ERas protein
• Target NameGTPase ERas
• Alternative Names    HRAS2, HRASP, ERAS, Embryonic stem cell-expressed Ras, E-Ras
• Accession No. Swiss-Prot#: Q7Z444
• UniprotQ7Z444
• Gene ID3266;
• Calculated MW 26kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.

[1] "Role of Eras in promoting tumor-like properties in mouse embryonic stem cells."Takahashi K., Mitsui K., Yamanaka S.Nature 423:541-545(2003) [2] "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gen

• All lanes : GTPase ERas antibody at 2ug/ml+293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size :26kDa Observed band size:26 kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting