• Product NameCaspase-7 Polyclonal Antibody;
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Caspase-7 polyclonal antibody;.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Caspase-7 protein
• Target NameCaspase-7;
• Alternative Names    Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic protease 3
• Accession No. Swiss-Prot#: P55210
• UniprotP55210
• Gene ID840;
• Calculated MW 34kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

[1] "Mch3, a novel human apoptotic cysteine protease highly related to CPP32."Fernandes-Alnemri T., Takahashi A., Armstrong R.C., Krebs J., Fritz L.C., Tomaselli K.J., Wang L., Yu Z., Croce C.M., Salveson G., Earnshaw W.C., Litwack G., Alnemri E.S.Cancer

• All lanes:Caspase-7 antibody at 2ug/ml Lane 1:A549 whole cell lysate Lane 2:HGC-27 whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :34kDa observed band size :34kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting