• Product NameEFTUD2 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen Affinity Purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total EFTUD2 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human 116 kDa U5 small nuclear ribonucleoprotein component protein (1-205aa)
• Target NameEFTUD2
• Alternative Names    Elongation factor Tu GTP-binding domain-containing protein 2, SNU114 homolog, hSNU114, U5 snRNP-specific protein, 116 kDa, U5-116 kDa, EFTUD2, KIAA0031, SNRP116
• Accession No. Swiss-Prot#: Q9GZT9
• UniprotQ9GZT9
• Gene ID54583;
• Calculated MW 109kd
• Concentration 1.0mg/mL
• Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:5000
  

Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex required for pre-mRNA splicing. Binds GTP.

[1]"Haploinsufficiency of a spliceosomal GTPase encoded by EFTUD2 causes mandibulofacial dysostosis with microcephaly."Lines M.A., Huang L., Schwartzentruber J., Douglas S.L., Lynch D.C., Beaulieu C., Guion-Almeida M.L., Zechi-Ceide R.M., Gener B., Gillessen-Kaesbach G., Nava C., Baujat G., Horn D., Kini U., Caliebe A., Alanay Y., Utine G.E., Lev D. Boycott K.M.Am. J. Hum. Genet. 90:369-377(2012). [2]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012). [3]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. Signal. 4:RS3-RS3(2011).

• All lanes:116 kDa U5 small nuclear ribonucleoprotein component antibody at 3ug/ml Lane 1:Hela whole cell lysate Lane 2:293T whole cell lysate Lane 3:NIH3T3 whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :109kDa observed band size :109kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting