• Product NamePTRH2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PTRH2 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human PTRH2
• Target NamePTRH2
• Alternative Names    BIT1; PTH2; CFAP37; CGI-147
• Accession No. Swiss-Prot#: Q9Y3E5
  Gene ID: 51651
• UniprotQ9Y3E5
• Gene ID51651;
• Calculated MW 19kd
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:25-1:100

PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1 (Bcl-2 inhibitor of transcription 1), is a 179 amino acid mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondia to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. It is also believed that the natural substrate for PTRH2 may be petidyl-tRNAs, which leave the ribosomes during protein synthesis.?

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-5: Raji cells, Jurkat cells, 231 cells, hepg2 cells, K562 cells
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #42725 at dilution 1/20,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting