• Product NameSMARCB1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SMARCB1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human SMARCB1
• Target NameSMARCB1
• Alternative Names    RDT; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144
• Accession No. Swiss-Prot#: Q12824
  Gene ID: 6598
• UniprotQ12824
• Gene ID6598;
• Calculated MW 44kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.?

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Hepg2 cells, K562 cells, Jurkat cells
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #42763 at dilution 1/30.
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #42763 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting