• Product NameVPS28 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total VPS28 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human VPS28
• Target NameVPS28
• Alternative Names    ESCRT-I complex subunit VPS28; H-Vps28; MGC60323; vacuolar protein sorting 28 homolog (S. cerevisiae); Vacuolar protein sorting-associated protein 28 homolog
• Accession No. Swiss-Prot#: Q9UK41
  Gene ID: 51160
• UniprotQ9UK41
• Gene ID51160;
• Calculated MW 25kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane: Human fetal liver tissue
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human Breast cancer tissue using #42836 at dilution 1/100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting