• Product NameNDUFB3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NDUFB3 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human NDUFB3
• Target NameNDUFB3
• Alternative Names    B12; CI-B12
• Accession No. Swiss-Prot#: O43676?
  Gene ID: 4709
• UniprotO43676
• Gene ID4709;
• Calculated MW 11kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Hela, PC3 and 293T cell
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #42882 at dilution 1/20,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting