• Product NamePSMF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PSMF1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human PSMF1
• Target NamePSMF1
• Alternative Names    PI31
• Accession No. Swiss-Prot#: Q92530
  Gene ID: 9491
• UniprotQ92530
• Gene ID9491;
• Calculated MW 30kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: Hela and HEPG2 cell
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 35 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #42932 at dilution 1/30,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting