• Product NameEEF1E1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total EEF1E1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human EEF1E1
• Target NameEEF1E1
• Alternative Names    P18; AIMP3
• Accession No. Swiss-Prot#: O43324
  Gene ID: 9521
• UniprotO43324
• Gene ID9521;
• Calculated MW 20kd
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-6: A431 cell£¬Hepg2 cell£¬293T cell£¬Human testis tissue£¬Jurkat and A549 cell
  Primary antibody: 1/300 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 2 seconds
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #42964 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #42964 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting