• Product NameIPO5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total IPO5 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human IPO5
• Target NameIPO5
• Alternative Names    IMB3; Pse1; imp5; KPNB3; RANBP5
• Accession No. Swiss-Prot#: O00410
  Gene ID: 3843
• UniprotO00410
• Gene ID3843;
• Calculated MW 124kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Hela, 293T and hepg2 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting