• Product NameWASL Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total WASL protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human WASL
• Target NameWASL
• Alternative Names    NWASP; N-WASP
• Accession No. Swiss-Prot#: O00401
  Gene ID: 8976
• UniprotO00401
• Gene ID8976;
• Calculated MW 55kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-4: K562, hela, A172 and 231 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43021 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43021 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting