• Product NameGCLM Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GCLM protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human GCLM
• Target NameGCLM
• Alternative Names    GLCLR
• Accession No. Swiss-Prot#: P48507
  Gene ID: 2730
• UniprotP48507
• Gene ID2730;
• Calculated MW 31kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.?

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Lovo, MCF7 and hela cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43024 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43024 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting