• Product NameHPRT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HPRT1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human HPRT1
• Target NameHPRT1
• Alternative Names    HPRT; HGPRT
• Accession No. Swiss-Prot#: P00492
  Gene ID: 3251
• UniprotP00492
• Gene ID3251;
• Calculated MW 25kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-4: Hela, 231, hepg2 and A549 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43033 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43033 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting