• Product NameCD14 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total CD14 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human CD14
• Target NameCD14
• Alternative Names    CD14 antigen; CD14 molecule; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form;
• Accession No. Swiss-Prot#: P08571
  Gene ID: 929
• UniprotP08571
• Gene ID929;
• Calculated MW 40kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.?

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: A549 and NIH/3T3 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43038 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43038 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting