• Product NameBTAF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total BTAF1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human BTAF1
• Target NameBTAF1
• Alternative Names    MOT1; TAF172; TAFII170; TAF(II)170
• Accession No. Swiss-Prot#: O14981
  Gene ID: 9044
• UniprotO14981
• Gene ID9044;
• Calculated MW 207kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-5: NIH/3T3, hepg2, hela, 231 and Raji cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43040 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43040 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting