• Product NameSF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SF1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human SF1
• Target NameSF1
• Alternative Names    BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
• Accession No. Swiss-Prot#: Q15637
  Gene ID: 7536
• UniprotQ15637
• Gene ID7536;
• Calculated MW 68kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: Hepg2 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 3 minutes
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43060 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43060 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting