• Product NamePOLE3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total POLE3 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human POLE3
• Target NamePOLE3
• Alternative Names    p17; YBL1; CHRAC17; CHARAC17
• Accession No. Swiss-Prot#: Q9NRF9
  Gene ID: 54107
• UniprotQ9NRF9
• Gene ID54107;
• Calculated MW 17kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: Hepg2 and MCF7 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43062 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43062 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting