• Product NameEEF1B2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total EEF1B2 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human EEF1B2
• Target NameEEF1B2
• Alternative Names    EF1B; EEF1B; EEF1B1
• Accession No. Swiss-Prot#: P24534
  Gene ID: 1933
• UniprotP24534
• Gene ID1933;
• Calculated MW 25kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Hela, hepg2 and 293T cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43073 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43073 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting