• Product NameNHLRC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NHLRC1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human NHLRC1
• Target NameNHLRC1
• Alternative Names    EPM2A; EPM2B; MALIN; bA204B7.2
• Accession No. Swiss-Prot#: Q6VVB1
  Gene ID: 378884
• UniprotQ6VVB1
• Gene ID378884;
• Calculated MW 42kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a single subunit E3 ubiquitin ligase. Laforin is polyubiquitinated by the encoded protein. Defects in this intronless gene lead to an accumulation of laforin and onset of Lafora disease, also known as progressive myoclonic epilepsy type 2 (EPM2).

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Lo2 cell
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 3 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting