• Product NamePSMB8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PSMB8 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human PSMB8
• Target NamePSMB8
• Alternative Names    JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
• Accession No. Swiss-Prot#: P28062
  Gene ID: 5696
• UniprotP28062
• Gene ID5696;
• Calculated MW 30kd
• Concentration 2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-6: Hela cells, Raji cells, Jurkat cells, Human placenta tissue, A431 cells, 231 cells,
  Primary antibody: 1/350 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #43134 at dilution 1/30.
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43134 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting