• Product NameRCC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RCC1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human RCC1
• Target NameRCC1
• Alternative Names    CHC1; RCC1-I; SNHG3-RCC1
• Accession No. Swiss-Prot#: P18754
  Gene ID: 1104
• UniprotP18754
• Gene ID1104;
• Calculated MW 45kd
• Concentration 1.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:30-1:150

RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity and functions as a GEF inhibitor, deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: HepG2 cells
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting