• Product NameCreatine Kinase BB antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 165 and 357 of Creatine Kinase BB
• Target NameCreatine Kinase BB
• Alternative Names    B-CK; CKBB
• Accession No. Swiss-Prot:P12277
  Gene ID:1152
• UniprotP12277
• Gene ID1152;
• Concentration 0.5mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  Immunofluorescence: 1:100-1:200
  

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

• Sample(30 ug of whole cell lysate)
  A: H1299
  B: HeLa S3
  12% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain) antibody at 1: 100 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB antibody at 1: 100 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting