• Product NameTPD52 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TPD52 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human TPD52
• Target NameTPD52
• Alternative Names    D52; N8L; PC-1; PrLZ; hD52
• Accession No. Swiss-Prot#: P55327
  Gene ID: 7163
• UniprotP55327
• Gene ID7163;
• Calculated MW 24kd
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The tumor protein D52 (TPD52) family consists of three members, TPD52, TPD52L1 (D53), and TPD52L2 (D54). These small coiled-coil motif bearing proteins interact in hetero- and homomeric fashion. The TPD52 gene maps to chromosome 8q21, and due to amplification, shows frequent overexpression in prostate and breast carcinomas. TPD52 bound to annexin VI in a Ca(2+)-dependent manner, suggesting that these molecules may act in concert to regulate secretory processes in plasma cells.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-4: Raji cells, 231 cells, 293T cells, A549 cells
  Primary antibody: 1/320 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting