• Product NameMKK3(Phospho-Ser189) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of MKK3 only when phosphorylated at serine189.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3.
• Target NameMKK3
• ModificationPhospho
• Alternative Names    MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3
• Accession No. Swiss-Prot: P46734
  NCBI Protein: NP_002747.2
• UniprotP46734
• Gene ID5606;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 40kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

• Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) Antibody #11145.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Phospho-Ser189) Antibody #11145(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Phospho-Ser189) Antibody #11145.
• Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MKK3 (Phospho-Ser189) Antibody #11145.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting