• Product NameAP2A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total AP2A1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human AP2A1
• Target NameAP2A1
• Alternative Names    ADTAA; CLAPA1; AP2-ALPHA
• Accession No. Swiss-Prot#: O95782
  Gene ID: 160
• UniprotO95782
• Gene ID160;
• Calculated MW 108kd
• Concentration 1.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:10-1:50

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: A431, 231 and NIH/3T3 cell
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 15 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43215 at dilution 1/20,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting