• Product NameATG16L1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ATG16L1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human ATG16L1
• Target NameATG16L1
• Alternative Names    IBD10; WDR30; APG16L; ATG16A; ATG16L
• Accession No. Swiss-Prot#: Q676U5
  Gene ID: 55054
• UniprotQ676U5
• Gene ID55054;
• Calculated MW 68kd
• Concentration 3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: Hela and raji cell
  Primary antibody: 1/750 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43247 at dilution 1/45.
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43247 at dilution 1/45.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting