• Product NameCOX7C Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total COX7C protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human COX7C
• Target NameCOX7C
• Alternative Names    COX7C; Cytochrome c oxidase polypeptide VIIc; Cytochrome c oxidase subunit 7C, mitochondrial; cytochrome c oxidase subunit VIIc
• Accession No. Swiss-Prot#: P15954
  Gene ID: 1350
• UniprotP15954
• Gene ID1350;
• Calculated MW 7kd
• Concentration 2.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-7: Mouse muscle and human fetal muscle tissue, mouse heart tissue and PC3 cell, mouse kidney and small intestines tissue, 231 cell
  Primary antibody: 1/300 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43250 at dilution 1/40.
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #43250 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting