• Product NameUBE2I Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total UBE2I protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human UBE2I
• Target NameUBE2I
• Alternative Names    P18; UBC9; C358B7.1
• Accession No. Swiss-Prot#: P63279?
  Gene ID: 7329
• UniprotP63279
• Gene ID7329;
• Calculated MW 18kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.?

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: TM4, hela and 293T cell
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human Colorectal tissue using #43252 at dilution 1/200.
• Immunohistochemical analysis of paraffin-embedded Human Lung tissue using #43252 at dilution 1/200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting