• Product NameCLDN25 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CLDN25 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human CLDN25
• Target NameCLDN25
• Alternative Names    C9JDP6; claudin-like; hCG1646420; LOC644672
• Accession No. Swiss-Prot#: C9JDP6
  Gene ID: 644672
• UniprotC9JDP6
• Gene ID644672;
• Calculated MW 25kd
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:100-1:200

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.?

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane: Human normal lung tissue
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human Colorectal cancer tissue using #43254 at dilution 1/200.
• Immunohistochemical analysis of paraffin-embedded Human Cervica cancer tissue using #43254 at dilution 1/200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting