• Product NameE2F5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total E2F5 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human E2F5
• Target NameE2F5
• Alternative Names    E2F-5
• Accession No. Swiss-Prot#: Q15329
  Gene ID: 1875
• UniprotQ15329
• Gene ID1875;
• Calculated MW 38kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: K562 cell and mouse brain tissue
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43261 at dilution 1/25,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting