• Product NameGPC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GPC1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human GPC1
• Target NameGPC1
• Alternative Names    glypican
• Accession No. Swiss-Prot#: P35052
  Gene ID: 2817
• UniprotP35052
• Gene ID2817;
• Calculated MW 62kd
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: HEPG2 and Jurkat cell
  Primary antibody: 1/350 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 40 seconds
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43277 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43277 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting