• Product NameRNF40 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RNF40 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human RNF40
• Target NameRNF40
• Alternative Names    BRE1B; RBP95; STARING
• Accession No. Swiss-Prot#: O75150
  Gene ID: 9810
• UniprotO75150
• Gene ID9810;
• Calculated MW 114kd
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Hela cells, 231 cells, A172 cells
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43326 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #43326 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting