• Product NameMED28 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MED28 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFull length fusion protein of human MED28
• Target NameMED28
• Alternative Names    EG1; magicin; 1500003D12Rik
• Accession No. Swiss-Prot#: Q9H204
  Gene ID: 80306
• UniprotQ9H204
• Gene ID80306;
• Calculated MW 20kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Med28 (mediator complex subunit 28), also known as EG1 (endothelial-derived protein 1), magicin (merlin and Grb2-interacting cytoskeletal protein) or tumor angiogenesis marker EG-1, is a 178 amino acid subunit of the Mediator complex that localizes to both nucleus and cytoplasm.?

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane: Human placenta tissue
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43339 at dilution 1/30.
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43339 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting