• Product NameUBE2J2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total UBE2J2 protein.
• Immunogen DescriptionFusion protein of human UBE2J2
• Target NameUBE2J2
• Alternative Names    NCUBE2; NCUBE-2; PRO2121
• Accession No. Swiss-Prot#: Q8N2K1
  Gene ID: 118424
• UniprotQ8N2K1
• Gene ID118424;
• Calculated MW 29kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:20-1:100

   

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: 293T cell,Mouse skeletal muscle tissue and HEPG2 cell
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 3 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43366 at dilution 1/20,

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting