• Product NameKDM6B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total KDM6B protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human KDM6B
• Target NameKDM6B
• Alternative Names    JMJD3
• Accession No. Swiss-Prot#: O15054
  Gene ID: 23135
• UniprotO15054
• Gene ID23135;
• Calculated MW 177kd
• Concentration 2.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

KDM6B (Lysine demethylase 6B) is a 1,679 amino acid nuclear protein that contains one JmjC domain and belongs to the highly conserved JmjC domain-containing protein family. Functioning as a histone demethylase, JMJD3 uses iron and ascorbate as cofactors to demethylate dimethylated and trimethylated Lys-27 residues of Histone H3, thereby playing an important role in the modification of the histone code.?

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane: A172 cells
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting