• Product NameSMYD5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SMYD5 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human SMYD5
• Target NameSMYD5
• Alternative Names    RRG1; RAI15; NN8-4AG; ZMYND23
• Accession No. Swiss-Prot#: Q6GMV2?
  Gene ID: 10322
• UniprotQ6GMV2
• Gene ID10322;
• Calculated MW 47kd
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

The SMYD (SET and MYND domain) family of lysine methyltransferases (KMTs) plays pivotal roles in various cellular processes, including gene expression regulation and DNA damage response.?SMYD1, SMYD2, and SMYD3 share a high degree of sequence homology and, with the exception of SMYD5, human SMYD proteins harbor at least one C-terminal tetratrico peptide repeat (TPR) domain. The SMYD proteins have been found to be linked to various cancers and biological processes.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-4: HT29 cells, 231 cells, hela cells, A549 cells
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting