• Product NameSMYD2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total SMYD2 protein.
• Immunogen DescriptionSynthetic peptide of human SMYD2
• Target NameSMYD2
• Alternative Names    KMT3C; HSKM-B; ZMYND14
• Accession No. Swiss-Prot#: Q9NRG4
  Gene ID: 56950
• UniprotQ9NRG4
• Gene ID56950;
• Calculated MW 50kd
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:200

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Mouse heart tissue
  Primary antibody: 1/350 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human Colorectal cancer tissue using #43434 at dilution 1/200.
• Immunohistochemical analysis of paraffin-embedded Human Lung cancer tissue using #43434 at dilution 1/200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting