• Product NameARFGAP2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total ARFGAP2 protein.
• Immunogen DescriptionSynthetic peptide of human ARFGAP2
• Target NameARFGAP2
• Alternative Names    IRZ; ZFP289; ZNF289; NBLA10535
• Accession No. Swiss-Prot#: Q8N6H7
  Gene ID: 84364
• UniprotQ8N6H7
• Gene ID84364;
• Calculated MW 57kd
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:100-1:200

ZNF289 (zinc finger protein 289), also known as ARFGAP2 (ADP-ribosylation factor GTPase activating protein 2), IRZ, Zfp289 or Nbla10535, functions as a GTPase-activating protein (GAP) for ARF family proteins. Localizing to the cytoplasmic side of the Golgi apparatus, ZNF289 contains one ARF-GAP domain and is found associated with COP-I-coated vesicles.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane 1-2: A431 and hela cell
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 15 seconds
• Immunohistochemical analysis of paraffin-embedded Human Colorectal cancer tissue using #43440 at dilution 1/100.
• Immunohistochemical analysis of paraffin-embedded Human Esophagus cancer tissue using #43440 at dilution 1/200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting