• Product NameCBX4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CBX4 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human CBX4
• Target NameCBX4
• Alternative Names    PC2; NBP16
• Accession No. Swiss-Prot#: O00257
  Gene ID: 8535
• UniprotO00257
• Gene ID8535;
• Calculated MW 61kd
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Human fetal brain tissue
  Primary antibody: 1/250 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting