• Product NameTUBB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TUBB protein.
• Immunogen DescriptionFull length fusion protein
• Target NameTUBB
• Alternative Names    M40; TUBB1; TUBB5; CDCBM6; OK/SW-cl.56
• Accession No. Swiss-Prot#: P07437
  NCBI Gene ID: 203068
• UniprotP07437
• Gene ID203068;
• Calculated MW 50kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane 1-5: NIH/3T3£¬HEPG2£¬Hela and 293T cell£¬Mouse brain tissue lysates,
  Primary antibody:TUBB antibody at dilution 1/300,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 10 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TUBB Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TUBB Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting